Indirect Assessment of Hydroxymethylglutaryl-CoA Reductase (NADPH) Activity in Liver Tissue

摘要: We describe an indirect method for assessing variation in 3-hydroxy-3-methylglutaryl-coenzyme A reductase (NADPH) activity liver tissue. 3-Hydroxy-3-methylglutaryl-CoA and mevalonate concentrations the tissue homogenate are estimated terms of absorbances ratio between two is taken as index enzyme, which catalyzes conversion 3-hydroxy-3-methylglutaryl-CoA to mevalonate. The increases under conditions this enzyme reportedly decreases (e.g., fasting, cholesterol feeding) Triton injection, phenobarbital treatment). assessed magnitude hepatic cholesterogenesis intact male albino rats by using [1,2-14C] acetate, fed controls, fasted, cholesterol-fed, Triton-treated, phenobarbital-treated. ratio, 3-hydroxy-3-methylglutarylCoA/mevalonate was all above groups. simple, reproducible, does not require any unusual chemicals.