Biological roles of the major capsid proteins and relationships between the two existing serotypes of infectious bursal disease virus.

摘要: Neutralizing monoclonal antibodies (n-MAbs) were produced against infectious bursal disease virus (IBDV) of serotypes 1 and 2. The n-MAbs recognizing the major antigenic proteins VP2 VP3, characterized using different strains IBDV representing existing two a variant subtype serotype 1. biological properties these viral antigens as defined by MAbs in vitro, studied utilizing post-adsorption neutralization tests fluorescence-activated cell sorter analysis. directed immunodominant epitopes on capable enhanced but did not inhibit attachment to susceptible cells. These able neutralize interfering with an event subsequent adsorption, possibly inhibiting penetration or uncoating. On contrary, MAb that immunoprecipitated other capsid protein VP3 was prevent although it possessed lower titers. Cross-immunoprecipitations various antisera revealed interrelationships between IBDV.