Characterization of the antigenic, immunogenic, and pathogenic variation of infectious bursal disease virus due to propagation in different host systems (bursa, embryo, and cell culture). II. Antigenicity at the epitope level.

摘要: Differences in the relative pathogenicity of variant (1084 E and GLS) standard (Edgar STC) infectious bursal disease virus (IBDV) strains were observed after propagation bursa Fabricius, embryos, or cell cultures. Bursa-derived IBDV induced most severe lesions Fabricius when compared with propagated embryos Embryo-derived moderate gross lesions, whereas culture-derived did not damage grossly. A high frequency re-isolations was obtained from bursal, spleen, thymic samples collected birds inoculated bursa-derived embryo-derived IBDV. Virus re-isolation occurred much less frequently culture-adapted Serological evaluations demonstrated that a higher neutralizing antibody response than strains. These results document immunogenicity is reduced following