Apoptosis is induced by infectious bursal disease virus replication in productively infected cells as well as in antigen-negative cells in their vicinity

摘要: The kinetics of infectious bursal disease virus (IBDV) replication and induction apoptosis were investigated in vitro vivo. After infection chicken embryo (CE) cells with IBDV strain Cu-1, the proportion apoptotic increased from 5·8% at 4 h post-infection (p.i.) to 64·5% 48 h p.i. correlated replication. UV-inactivated particles did not induce apoptosis. Double labelling revealed that, early after infection, majority antigen-expressing apoptotic; double-labelled appeared more frequently later times. Remarkably, located vicinity cells. This indicated that an apoptosis-inducing factor(s) might be released by replicate IBDV. Since interferon (IFN) production has been demonstrated IFN was considered one several factors. However, supernatants infected CE which infectivity had neutralized sufficient apoptosis.Similar results observed bursae Fabricius: most either showed antigens or apoptotic. Again, late infection. suggests indirect mechanisms also involved vivo, contributing rapid depletion IBDV-infected bursa.