Pathogenic and structural properties of wild type infectious bursal disease virus (IBDV) and virus grown in vitro.

摘要: Large plaque (LP) and small (SP) variants of Infectious Bursal Disease Virus (IBDV) which are formed in vitro after serial passages CE-cells at low or high multiplicities infection were tested for their pathogenic properties susceptible chickens. LP virus caused clinical manifestations destruction the Bursa Fabricius (BF) without killing animals. No signs a disease appeared with SP virus, only limited necrotic foci developed BF. Coinfection variant highly wild type (wt) reduced mortality severity disease. In contrast to variant, forms incomplete particles density, one other two genomic RNA segments missing, characteristic IBDV present approximately equal amounts wt buoyant density 1.29 g/ml isolated from spite an almost total replacement major structural polypeptides molecular weight 32 kd by 28 protein, infectivity this fraction was slightly reduced. This finding underlines importance post-translational modification lymphoid cells fibroblasts.