Replication of infectious bursal disease virus in macrophages and altered tropism of progeny virus.
作者： Mahesh Khatri , Jagdev M. Sharma
DOI: 10.1016/J.VETIMM.2007.02.002
关键词:
摘要: We serially passaged classical infectious bursal disease virus (cIBDV) and antigenic variant IBDV (vIBDV) in an avian macrophage cell line, NCSU cells, referred as mcIBDV mvIBDV respectively examined the vitro vivo characteristics of macrophage-adapted viruses. adapted viruses caused earlier destruction cells than unadapted Nitric oxide (NO) was detected cultures infected with cIBDV vIBDV. vIBDV were able to infect DF-1 a chicken embryo fibroblast only after one replication cycle cells. The genetic basis altered tropism progeny from not identified. No aa substitutions observed hypervariable region VP2 1 time whereas both had multiple substitutions. To assess protective efficacy mvIBDV, embryonated eggs inoculated at embryonation day 18 (ED 18) challenged virulent 3 weeks age. immunogenic generated antibody responses provided 100% protection against cIBDV.
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