Arabidopsis genes, AtNPR1, AtTGA2 and AtPR-5 , confer partial resistance to soybean cyst nematode ( Heterodera glycines ) when overexpressed in transgenic soybean roots

摘要: Extensive studies using the model system Arabidopsis thaliana to elucidate plant defense signaling and pathway networks indicate that salicylic acid (SA) is key hormone triggering response against biotrophic hemi-biotrophic pathogens, while jasmonic (JA) derivatives are critical necrotrophic pathogens. Several reports demonstrate SA limits nematode reproduction. Here we translate knowledge gained from soybean. The ability of thirty-one genes encoding important components JA synthesis in conferring resistance soybean cyst (SCN: Heterodera glycines) investigated. We overexpression three Arabidoposis transgenic roots composite plants decreased number cysts formed by SCN less than 50% those found on control roots, namely AtNPR1(33%), AtTGA2 (38%), AtPR-5 (38%). Three additional 40% or more: AtACBP3 (53% value), AtACD2 (55%), AtCM-3 (57%). Other having no effect included AtEDS5 (77%), AtNDR1 (82%), AtEDS1 (107%), AtPR-1 (80%), as compared control. Overexpression AtDND1 greatly increased susceptibility indicated a large increase (175% control). Knowledge pathogen system, Arabidopsis, can be directly translated through direct genes. When genes, AtNPR1, AtGA2, AtPR-5, specific involved regulation, synthesis, signaling, overexpressed enhanced. This demonstrates functional compatibility some with identifies may used engineer nematodes.