B lineage--specific interactions of an immunoglobulin enhancer with cellular factors in vivo
作者： A Ephrussi , G. Church , S Tonegawa , W Gilbert
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摘要: The mouse heavy chain immunoglobulin gene contains a tissue-specific enhancer. enhancer and flanking sequences were studied in vivo by carrying out dimethyl sulfate protection experiments on living cells, combination with genomic sequencing. Relative to reactions naked DNA, there are changes (protections enhancements) the reactivity of guanine residues within sequence myeloma, B, early B whereas virtually no alterations appear cells non-B lineage. Most affected four clusters, homologous octamer 5'CAGGTGGC 3' (C, cytosine; A, adenine; G. guanine; T, thymine). pattern G consistent binding molecules
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