Mutation of the PTEN (MMAC1) Tumor Suppressor Gene in a Subset of Glioblastomas but not in Meningiomas with Loss of Chromosome Arm 10q

摘要: The PTEN (MMAC1) gene, which has been identified as a tumor suppressor gene at 10q23.3, is mutated in multiple malignant tumors, including glioblastomas [J. Li et al., Science (Washington DC), 275: 1943-1947, 1997; P. A. Steck Nat. Genet., 15: 356-362, 1997]. Among tumors of the central nervous system, loss 10q not restricted to but also common atypical and anaplastic meningiomas. Therefore, we have investigated 36 34 meningiomas (2 benign, 17 atypical, 15 meningiomas) for on 10q, well deletion, mutation, expression PTEN. Analysis eight microsatellites from revealed heterozygosity (LOH) 25 (69%). Twenty-three these demonstrated LOH all informative loci. Two showed markers located distally PTEN, with breakpoints mapping telomeric D10S541 D10S185. One glioblastoma evidence homozygous deletion by differential PCR analysis. mutations were detected 9 (25%). Seven loci indicating complete wild-type Although was comparative genomic hybridization and/or analysis 14 (41%), none or deletions. Our findings corroborate that inactivated subset glioblastomas. However, lack detectable alterations considerable fraction strongly supports hypothesis least one additional 10q.