Effects of midazolam on brain injury after transient focal cerebral ischemia in rats

摘要: The benzodiazepine, midazolam, is commonly used for sedation and anesthesia in the operating room intensive care unit where there a risk of cerebral ischemia. We therefore examined its ability to reduce damage subsequent Male Wistar rats were randomly assigned high-dose midazolam group or matched vehicle lower dose group. underwent 90 minutes middle artery occlusion. In groups, first (10 25 mg/kg) was given by 10-minute intravenous infusion before ischemia; second (1/2) initial (5 12.5 1 hour after onset similar volume at same time intervals. Infarct size, NeuN immunopositive cells ischemic penumbral core regions, neurologic outcome determined 7 days Compared with vehicle-treated rats, higher-dose (25 mg/kg)-treated had smaller infarct size (93.9+/-63.5 mm vs. 152.0+/-53.7 mm, P<0.05), more region (206.7+/-211.3/mm 40.0+/-66.3/mm, P<0.01), better (P<0.05). Midazolam no significant effects. Although generated dose-dependent hemolysis, this hemolysis transient. caused loss righting reflex that lasted until 19.9+/-1.3 min injection, anesthetic approximately 100x greater than humans. An reduced neuronal improved focal ischemia, however, it also transient hemolysis.