Effects of ketamine, midazolam, thiopental, and propofol on brain ischemia injury in rat cerebral cortical slices.
作者： Bu-wei Yu , Ze-jian Wang , Hong-zhuan Chen , Qing-sheng Xue
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摘要: AIM: To compare the effects of ketamine, midazolam, thiopental, and propofol on brain ischemia by model oxygen-glucose deprivation (OGD) in rat cerebral cortical slices. METHODS: Cerebral slices were incubated 2 % 2,3,5-triphenyltetrazolium chloride (TTC) solution after OGD, damages quantitatively evaluated ELISA reader absorbance (A) at 490 nm, which indicated red formazan extracted from slices, lactic dehydrogenase (LDH) releases supernate also measured. RESULTS: Progressive prolongation OGD resulted decreases TTC staining. The percentage tissue injury had a positive correlation with LDH releases, r=0.9609, P<0.01. Two hours reincubation aggravated decrease staining compared those stained immediately (P<0.01). These four anesthetics no slices. Ketamine completely inhibited A value induced 10 min injury. High concentrations midazolam (10 micromol/L) thiopental (400 partly attenuated this decrease. Propofol high concentration (100 enhanced (P<0.01). CONCLUSION: Ketamine, have neuroprotective against while augments
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