Identification of Edc3p as an Enhancer of mRNA Decapping in Saccharomyces cerevisiae

摘要: The major pathway of mRNA decay in yeast initiates with deadenylation, followed by decapping and 5′-3′ exonuclease digestion. An silico approach was used to identify new proteins involved the pathway. One such protein, Edc3p, identified as a conserved protein unknown function having extensive two-hybrid interactions several degradation including Dcp1p, Dcp2p, Dhh1p, Lsm1p, exonuclease, Xrn1p. We show that Edc3p can stimulate both unstable stable mRNAs when enzyme is compromised temperature-sensitive alleles either DCP1 or DCP2 genes. In these cases, deletion EDC3 caused synergistic mRNA-decapping defect at permissive temperatures. edc3 Δ had no effect combined lsm1 Δ, dhh1 pat1 mutations, which appear affect an early step This suggests specifically affects per se . Consistent functional role decapping, GFP-tagged localizes cytoplasmic foci referred P-bodies. These results reaction.