摘要: Publisher Summary This chapter discusses fixation and staining. Cytochemistry with the electron microscope suffers from all defects of cytochemistry light microscope. With microscope, specificity, sensitivity, diffusion, are major problems. Fixation can be defined as selective preservation cell or tissue structures components for subsequent study. is in sense that it selects those methods most suitable particular purpose research The essential effect separation solid phase protoplasm aqueous phase. Every cytochemical procedure requires substance should treated if solution, yet must remain insoluble not shift more than a few Angstrom units. There certain procedures simpler microscopy microscopy. same reagents concentrations used biochemically identification groups work.
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