Phosphorescent detection of DNA- drug interaction based on emission quenching of ZnS quantum dots via photoinduced electron transfer

摘要: Abstract A novel room temperature phosphorescence (RTP) sensor consisting of quantum dots (QDs)-drug nanohybrid was used for the investigation interaction between epirubicin (EPI) and double-stranded deoxyribonucleic acid (ds-DNA). The method based on quenching effect EPI emission Mn-doped ZnS QDs via photoinduced electron-transfer (PIET) mechanism. Adsorption to surface caused RTP process. Whereas, with addition DNA provided restoration due removing from surface. were synthesized in an aqueous medium characterized. diameter prepared about 3.5 nm, spherical, uniform size. mechanism by is not only collisional but also static. static dynamic constants found as 5.36 × 105 M−1 3.19 104 M−1, respectively. In this method, fluorescence absorption spectrometric methods evaluate DNA/drug calculate binding constant (K), which 3.83 M−1. Proposed has advantages such simplicity avoids interferences autofluorescence scattering light.