Distribution of Native Lactic Acid Bacteria in Wineries of Queretaro, Mexico and Their Resistance to Wine-Like Conditions.

摘要: Native lactic acid bacteria (LAB) are capable of growing during winemaking, thereby strongly affecting wine quality. The species LAB present in musts, wines malolactic fermentation (MLF), and barrels/filters were investigated wineries from the emerging region Queretaro, Mexico using multiplex PCR culture. resistance to wine-like conditions (WLC): ethanol (10, 12 13%), SO2 (30 mg·l-1) low pH (3.5) native strains was also studied. Five detected within 61 samples obtained: Oenococcus oeni, Lactobacillus plantarum, Pediococcus parvulus, hilgardi brevis. Four (excepting L. brevis) found must; O. oeni P. parvulus ubiquitous plantarum brevis mainly at initial stage MLF, while hilgardii mostly advanced stage. Furthermore, some barrel/filter, sprove them be hazardous reservoirs. From 822 isolates, only 119 resisted WLC with 10% ethanol; number able grow 13% decreased approximately by 50%, being most versatile 65% resistant spp. affected, especially those recovered less than isolates. This study evidences presence local used as starter cultures, enabled assessment risks derived spoilage WLC.