DETERMINATION OF FIVE BIOACTIVE CONSTITUENTS IN TRADITIONAL CHINESE MEDICINAL PREPARATION SHANDANSHAOYAO TANG BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

摘要: A reversed phase high performance liquid chromatographic method for the determination of danshensu, protocatechuic acid, protocatechualdehyde, (+)-catechin, and paeoniflorin in traditional Chinese medicinal preparation Shandanshaoyao Tang was established. Danshensu, (+)-catechin were separated on a Diamonsil C18 column (250 mm × 4.6 I.D.) with mobile consisting methanol-acetic acid-water (14: 0.2: 86, v/v), detected by UV at 280 nm. Paeoniflorin using same but (25: 75, 230 The linear calibration ranges 24.1–241.0 μg/mL, 6.1–61.0 2.5–25.4 8.3–83.0 μg/mL 224–2235 paeoniflorin, respectively. relative standard deviations (n=5) average recoveries 1.45% 91.4% 1.54% 100.1% protoc...