Identification of the major autophosphorylation site of the Met/hepatocyte growth factor receptor tyrosine kinase.
作者： R. Ferracini , P. Longati , L. Naldini , E. Vigna , P.M. Comoglio
DOI: 10.1016/S0021-9258(18)55031-6
关键词:
摘要: The MET proto-oncogene encodes a transmembrane tyrosine kinase receptor for HGF (p190MET). In this work, p190MET was immunoprecipitated, allowed to phosphorylate in the presence of [gamma-32P]ATP, and digested with trypsin. A major phosphopeptide purified by reverse phase chromatography. phosphorylated identified as residue 1235 (Tyr1235) Edman covalent radiosequencing. synthetic peptide derived from corresponding sequence an vitro assay coeluted Tyr1235 lies within domain p190MET, canonical autophosphorylation site that shares homology region insulin, CSF-1 platelet-derived growth factor receptors, p60src p130gag-fps. is constitutively on tryosine gastric carcinoma cell line (GTL16), due amplification overexpression gene. Metabolic labeling GTL-16 cells [32P]orthophosphate followed immunoprecipitation tryptic mapping showed phosphorylation vivo well. Since activates kinase, we propose regulatory role Tyr1235.
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