Long-term culture of infant leukemia cells: dependence upon stromal cells from the bone marrow and bilineage differentiation.

摘要: Infant leukemia cells with 46XY,t(11; 17)(q23; p13) karyotype and a hybrid pre B myeloid phenotype (HLA-DR, (Ia), B4 My7-positive CALLA T11-negative) immunoglobulin heavy chain gene rearrangement were maintained in long-term culture for over 10 months. The in-vitro survival growth of the strictly dependent upon presence their autologous marrow stromal cells. latter could be replaced by 14F1.1 clone preadipocytes derived from mouse bone marrow. Neither heterologous human or foreskin fibroblasts nor fibroblast endothelial like cell lines stroma mimic effect adipocytes. original throughout 10-month period either They induced to differentiate two distinct directions. Phorbol myristate acetate adherence development macrophage properties. In contrast, conditioned medium hybridoma producing B-cell factor caused aggregation expression antigen surface IgM. This bipotency dependence are properties common stem