Selective determination of α2-plasmin inhibitor activity in plasma using chromogenic substrate

摘要: In determination of antiplasmin activity alpha 2-plasmin inhibitor (alpha 2 PI) using an amidolytic method, 2-macroglobulin M) in concentrations present normal plasma inhibits the cleavage chromogenic substrates by plasmin even if reaction time between 2M and is shortened as much possible. The purpose this study was to develop a new method assay PI selectively with substrate S-2251 end-point method. M destroyed addition methylamine hydrochloride. case congenital deficiency, determined previous without 20% plasma, whereas results obtained newly developed 5%. A statistically significant correlation levels single radial immunodiffusion observed samples collected from 40 healthy subjects 11 patients disseminated intravascular coagulation (DIC). two cases DIC, which markedly depleted were observed, activities measured higher than ones immunologically, described here accordance antigenicities PI.