Synthesis of a biodegradable polymer in gas expanded solution: effect of the process on cytocompatibility

摘要: The aim of this study was to investigate the feasibility eliminating organic solvent consumption for synthesis biodegradable poly(lactide ethylene oxide fumarate) (PLEOF). A gas expanded solution comprised high pressure carbon dioxide (CO2) and dichloromethane (DCM) or poly(ethylene glycol) (PEG) used as an alternative medium PLEOF. effect (50–150 bar), temperature (25–50 °C) amount DCM on yield, molecular weight thermal properties PLEOF examined. results 1H NMR ATR-FTIR analysis confirmed that it is possible conduct in a CO2 reaction acquire similar characteristics compared conventional method using neat DCM. comparable yield achieved process even when decreased only 14% method. Increasing from 50 150 bar had no significant yield. In addition, could be completely eliminated polymerisation by increasing °C 100 bar. Under these conditions, carried out PEG due melting point depression CO2. It is, therefore, viable benign can either substantially reduce eliminate volatile solvents. synthesised also crosslinked with diacrylate (PEGDA) non-toxic crosslinker produce injectable hydrogel. hydrogel prepared better mechanical less toxicity primary human osteoblast cells. This demonstrated have impact improving physical biological activity polymer.