Fabrication of interpenetrating polymer network to enhance the biological activity of synthetic hydrogels

摘要: Abstract A three dimensional porous hydrogel with suitable biological and mechanical properties are required for bone tissue engineering. Hydrogels of poly(lactic-ethylene oxide fumarate) (PLEOF), crosslinked poly(ethylene glycol)-diacrylate (PEG-da) have desirable properties, however, their application regeneration is limited due to the lack cell motif sites within structure. The aim this study was incorporate a naturally derived polymer such as gelatin into PLEOF hydrogels promote properties. Interpenetrating network (IPN) used an efficient technique acquire uniform mixture these two polymers. Additionally gas foaming agents were create pores average diameter 250 μm in IPN hydrogels. concentrations PEG-da optimized tune strength degradation compression modulus 500 kPa achieved fabricated 400 mg/ml PLEOF, 200 mg/ml 150 mg/ml gelatin. addition elevated by two-fold decreased energy loss 40%. result protein analysis demonstrated that substantially enhanced retention physically 3D structure hydrogel. More than 50% retained after weeks incubation simulated physiological environment. Preserving provides longer period time, which proliferation. In vitro studies showed primary human osteoblast cells adhered proliferated PLEOF-gelatin These results potential using