Phylogenetic distribution and membrane topology of the LytR-CpsA-Psr protein family

摘要: The bacterial cell wall is the target of many antibiotics and envelope constituents are critical to host-pathogen interactions. To combat resistance development virulence, a detailed knowledge individual factors involved essential. Members LytR-CpsA-Psr family envelope-associated attenuators relevant for β-lactam resistance, biofilm formation, stress tolerance, they suggested play role in maintenance. However, their precise function still unknown. This study addresses occurrence as well sequence-based characteristics proteins. A comprehensive list proteins was established, phylogenetic distribution clustering into subgroups determined. were present all Gram-positive organisms, except wall-deficient Mollicutes one strain Clostridiales. In contrast, majority Gram-negatives did not contain members. Despite high sequence divergence, domains different subclusters shared highly similar, predicted mixed a/β-structure, conserved charged residues. PhoA fusion experiments, using MsrR Staphylococcus aureus, confirmed membrane topology predictions extracellular location its domain. domain unique bacteria. presence diverse within might indicate functional differences, could explain variations phenotypes respective mutants reported. identified structural elements amino acids likely be important will help guide future studies