The Cell Wall-Targeting Antibiotic Stimulon of Enterococcus faecalis

摘要: Enterococcus faecalis is an opportunistic nosocomial pathogen that highly resistant to a variety of environmental insults, including intrinsic tolerance antimicrobials target the cell wall (CW). With goal determining CW-stress stimulon E. faecalis, global transcriptional profile OG1RF exposed ampicillin, bacitracin, cephalotin or vancomycin was obtained via microarrays. Exposure β-lactams ampicillin and resulted in fewest changes with 50 192 genes differentially expressed 60 min after treatment, respectively. On other hand, treatment bacitracin for affected expression of, respectively, 377 297 genes. Despite differences total number affected, all antibiotics induced very similar gene pattern overrepresentation encoding hypothetical proteins, followed by proteins associated envelope metabolism as well transport binding proteins. In particular, drug treatments, most notably vancomycin, apparent metabolic downshift based on repression involved translation, energy metabolism, binding. Only 19 were up-regulated conditions at both 30 time points. Among those genes, 4 (EF0026, EF0797, EF1533 EF3245) inactivated respective mutant strains characterized relation antibiotic virulence Galleria mellonella model. The phenotypes two these mutants, ΔEF1533 ΔEF3245, support further characterization potential candidates development novel preventive therapeutic approaches.