Microarray analysis reveals an inflammatory transcriptomic signature in peripheral blood for sciatica.

摘要: BACKGROUND Although the pathology of sciatica has been studied extensively, transcriptional changes in peripheral blood caused by have not characterized. This study aimed to characterize transcriptomic signature for sciatica. METHODS We used a microarray identify differentially expressed genes patients with compared that healthy controls, performed functional analysis reveal sciatica, and conducted network key contribute observed changes. The expression levels these were assessed qRT-PCR. RESULTS found 153 131 22 upregulated downregulated, respectively. A revealed (DEGs) strongly enriched inflammatory response or immunity. group genes, most which are related response, played role dysregulation DEGs. These Toll-like receptor 4, matrix metallopeptidase 9, myeloperoxidase, cathelicidin antimicrobial peptide, resistin 5, qRT-PCR validated higher transcript than controls. CONCLUSION characteristics serve as identified essential mRNA