Prevalence of Plasmodium falciparum transmission reducing immunity among primary school children in a malaria moderate transmission region in Zimbabwe

摘要: Malaria continues to cause alarming morbidity and mortality in more than 100 countries worldwide. Antigens the various life cycle stages of malaria parasites are presented immune system during natural infection it is widely recognized that after repeated exposure, adults develop partially protective immunity. Specific antigens immunity represent among most important targets for development vaccines. Immunity against transmission parasite represents an approach reduce believed become tool gradual elimination malaria. Development Plasmodium falciparum sexual was evaluated primary school children aged 6-16 years Makoni district Zimbabwe, area low modest transmission. screened by microscopy, rapid diagnostic tests finally using nested PCR. Plasma samples were tested antibodies recombinant Pfs48/45 Pfs47 ELISA. Corresponding serum used test P. reducing activity Anopheles stephensi An. gambiae mosquitoes membrane feeding assay. The prevalence diagnosed kit (Paracheck)™ 1.7%. However, randomly blood samples, 66% positive ELISA revealed (64% positivity at 1:500 dilution, selected 66 plasma samples) (mean A 405nm=0.53, CI=0.46-0.60) A405nm=0.91, CI=0.80-1.02); specific stages. mosquito assay demonstrated measurable ability Interestingly, 3 enhancement infectivity mosquitoes. These studies presence with age from a moderate malaria, provide further support exploit target such as blocking vaccine.