Two apparently nonrepeated epitopes on gametes of Plasmodium falciparum are targets of transmission-blocking antibodies.

摘要: One-site and two-site immunoradiometric assays have been developed against an antigen on gametocytes of Plasmodium falciparum, using monoclonal antibodies (Mabs) which block transmission the parasites to mosquitoes. Three such Mabs studied, each immunoprecipitates a complex three gamete surface proteins apparent Mr 260,000, 59,000, 53,000 from Triton X-100 extracts parasites. The showed that recognized one or other two distinct, nonrepeated epitopes target antigen(s). In one-site assay certain combinations interacted at appropriate concentrations enhance binding antigen. same synergize suppress infectivity