Three non-repeated transmission blocking epitopes recognized in the 21 kD surface antigen of zygotes-ookinetes of Plasmodium berghei.
作者： NEDNAPIS TIRAWANCHAI , ROBERT E. SINDEN
DOI: 10.1111/J.1365-3024.1990.TB00979.X
关键词: Biology 、 Immunology 、 Plasmodium falciparum 、 Plasmodium berghei 、 Linear epitope 、 Antigen 、 Antibody 、 Monoclonal antibody 、 Glycosylation 、 Epitope 、 Virology 、 Molecular biology
摘要: Summary Two-site and competitive ELISA have been developed against a surface antigen of zygotes-ookinetes Plasmodium berghei using monoclonal antibodies (MoAbs) which block transmission the parasite to mosquito. Three such MoAbs studied, each recognized protein an Mr 21 kD (Pbs21) immunoblot techniques. The assays showed that there are at least 3 single B-cell epitopes expressed in Pbs21. One epitope by MoAb 17.9 is conformation dependent bound it interfered with other (12.1 13.1) recognizing different, apparently linear epitope. Glycosylation might not be relevant binding any tested their respective epitopes.
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