Studies on the immunogenicity of a recombinant ookinete surface antigen Pbs21 from Plasmodium berghei expressed in Escherichia coli

摘要: SUMMARY Plasmodium berghei ookinete surface antigen (Pbs21), was produced as a fusion product with maltose binding protein (MBP) in Escherichia coli and used to induce transmission-blocking immunity mice. Specificity of induced antibody confirmed by Western blotting native Pbs21, the indirect immunofluorescent test on bloodfilms. Immunized mice were infected P. transmission Anopheles stephensi mosquitoes determined both intensity prevalence oocyst infections. Compared control group immunized MBP alone maximum blockade 66% recombinant MBP-Pbs21. Over nine experiments averaged only 33%. By comparison Pbs21 protein, which usually induces 90% blockade, our data suggests this bacterial system is less effective immunogen despite expressing epitopes recognized known monoclonal antibodies.