Effects of exogenous glucose on brain ischemia in ovine fetuses.

摘要: We examined the effects of prolonged moderate hyperglycemia with and without an additional rapid glucose injection on ischemic brain injury in fetus. Twenty-five ewes (117-124 d gestation) were assigned to one four groups: 1) glucose-infused fetuses exposed 30 min carotid artery occlusion followed by 48 h reperfusion (I/R-Glu, n = 8); 2) plus given 100 before (I/R-GluR, 4); 3) placebo-infused (I/R-PL, 4) sham (control, 5). After baseline measurements, infused (9-16 mg/kg/min) for after or treatment. The I/R-PL group received 0.9% NaCl. Brain pathologic outcome was determined. Serial sections stained Luxol fast blue-hematoxylin eosin scored white matter, cerebral cortical, hippocampal lesions. These areas graded scores 0 5, reflecting amount injury, where 0%, 1 1-25%, 2 26-50%, 3 51-75%, 4 76-95%, 5 96-100% area damaged. Comparisons cortex (CC), matter (WM), hippocampus (H) demonstrated that I/R-GluR (CC: 4.56 +/- 0.11, WM: 4.50 H: 3.44 0.48, mean SEM) had more (p < 0.05) damage than I/R-Glu 2.46 0.47, 1.97 0.37, 1.81 0.36) control 1.12 0.13, 0.82 0.34, 0.80 0.34) groups. greater control, but not 2.12 0.35, 2.20 0.44, 1.59 0.41) group. conclude exposure ischemia during does affect extent acute increase plasma concentration is extremely detrimental fetal brain.