Massively parallel DNA sequencing from routinely processed cytological smears.
作者： Laure Piqueret‐Stephan , Charles Marcaillou , Cécile Reyes , Aurélie Honoré , Mélanie Letexier
DOI: 10.1002/CNCY.21639
关键词:
摘要: BACKGROUND Data generated by next-generation sequencing technologies have a pivotal role in precision medicine. These high-throughput techniques are preferentially performed on fresh tissue, but there is an increasing need for protocols adapted to materials derived from formalin-fixed, paraffin-embedded tissue and cytology specimens. METHODS The aim of this work was show that cytological material collected archival smears processed routine diagnoses could be used massively parallel array-based genomic analysis further studies. RESULTS As proof concept, data obtained May-Grunwald Giemsa– Diff-Quik–stained were shown keeping with those matched frozen controls. CONCLUSIONS The quality DNA extracted routinely compatible the multitargeted large series genes interest methods such as whole-exome sequencing. Cancer Cytopathol 2016;124:241–53. © 2015 American Society.
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