Acceleration of the refolding of Arc repressor by nucleic acids and other polyanions

摘要: The refolding rate of the Arc repressor dimer can be accelerated 30-fold or more by negatively charged polymers including single-stranded and double-stranded DNA, RNA, polyvinylsulfate but not neutral positively polymers. salt-dependence polyanion-mediated process mutant studies indicate that electrostatic interactions are important in acceleration. Urea-dependence suggest is relatively unstructured transition state for polyanion-stimulated refolding. At low ionic strength, observed kinetics consistent with a model which denatured monomers bind rapidly nonspecifically to polyanion complete folding bound state.