TbENF is an essential TbTFIIB-interacting trypanosomatid-specific factor
作者： Kellie Whitecavage Solnoki , Allison H. Sing , Caitlyn J. Sofa , Russell Miller , Paulina A. Ogorzalek
DOI: 10.1016/J.MOLBIOPARA.2011.10.004
关键词:
摘要: Trypanosoma brucei, the causative agent of African Sleeping Sickness, is replete with unique biochemistry, including unusual features gene transcription. The parasite also contains over 4500 non-annotated genes, representing novel biochemistry yet to be explored. Using tandem affinity purification (TAP)-tagged TbTFIIB, we identified and subsequently confirmed, one T. brucei proteins, Tb11.02.4300, as a TbTFIIB-interacting protein. 49 kDa protein nuclear essential for variability determined by RNA interference studies; hence, nomenclature Essential Nuclear Factor (TbENF). TbENF shown interact DNA in sequence-independent fashion under conditions examined. Furthermore, bears motifs associated many eukaryotic transcription factors, such glutamine-rich region leucine zipper, specific trypanosomatids making it potentially attractive therapeutic target. Taken together, our results suggest role trypanosome
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