Amino acids influence the glucose uptake through GLUT4 in CHO-K1 cells under high glucose conditions.
作者： Radhakrishnan Selvi , Narayanasamy Angayarkanni , Begum Asma , Thiagarajan Seethalakshmi , Srinivasan Vidhya
DOI: 10.1007/S11010-010-0527-1
关键词:
摘要: According to studies earlier, amino acids have proven be antidiabetic, antiglycating, and anticataractogenic. The present study was explore whether as mixtures could enhance glucose uptake in CHO-K1 cells specifically. F-12K1 serum-free medium were exposed normal (7 mM) high (12, 17 27 the presence absence of mixture (AAM) varying concentration (2.5, 5 10 mM). mM AAM increased 2-deoxyglucose (2DG) at all significantly. There also a significant increase GLUT4 (glucose transporter) translocation revealed by flow cytometer. Addition found improve cell viability, which got altered cells. Amino had beneficial effect improving net utilization an additive with insulin.
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sci-hub.se 参考文章(61)
K. N. Sulochana, C. Indra, M. Rajesh, V. Srinivasan, S. Ramakrishnan, Beneficial role of amino acids in mitigating cytoskeletal actin glycation and improving F-actin content: in vitro. Glycoconjugate Journal. ,vol. 18, pp. 277- 282 ,(2001) , 10.1023/A:1013666829851
Rajesh M, Ramakrishnan S, Indra C, Sulochana Kn, Srinivasan, Amino acids differentially regulate insulin receptor tyrosine kinase and phosphatidyl inositol-3-OH-kinase activities in human monocytes exposed to high glucose concentration. Indian Journal of Biochemistry & Biophysics. ,vol. 42, pp. 13- 18 ,(2005)
S Lakshmi, R Punitham, T Arokiasamy, B Sukumar, S Ramakrishnan, None, Effect of oral supplementation of free amino acids in type 2 diabetic patients: a pilot clinical trial. Medical Science Monitor. ,vol. 8, ,(2002)
Craig Ricci, Chian Ju Jong, Stephen W. Schaffer, Proapoptotic and antiapoptotic effects of hyperglycemia: role of insulin signaling. Canadian Journal of Physiology and Pharmacology. ,vol. 86, pp. 166- 172 ,(2008) , 10.1139/Y08-021
Adelle C. F. Coster, Roland Govers, David E. James, Insulin stimulates the entry of GLUT4 into the endosomal recycling pathway by a quantal mechanism. Traffic. ,vol. 5, pp. 763- 771 ,(2004) , 10.1111/J.1600-0854.2004.00218.X
R R Traxinger, S Marshall, Role of amino acids in modulating glucose-induced desensitization of the glucose transport system. Journal of Biological Chemistry. ,vol. 264, pp. 20910- 20916 ,(1989) , 10.1016/S0021-9258(19)30022-5
Juleen R. Zierath, Anna Krook, Harriet Wallberg-Henriksson, Insulin action in skeletal muscle from patients with NIDDM Insulin Action. ,vol. 182, pp. 153- 160 ,(1998) , 10.1007/978-1-4615-5647-3_16
M.J. MacDonald, J.H. Kaysen, S.M. Moran, C.E. Pomije, Pyruvate dehydrogenase and pyruvate carboxylase. Sites of pretranslational regulation by glucose of glucose-induced insulin release in pancreatic islets. Journal of Biological Chemistry. ,vol. 266, pp. 22392- 22397 ,(1991) , 10.1016/S0021-9258(18)54585-3
H Demaegdt, L Smitz, J-P De Backer, M T Le, M Bauwens, E Szemenyei, G Tóth, Y Michotte, P Vanderheyden, G Vauquelin, Translocation of the insulin-regulated aminopeptidase to the cell surface: detection by radioligand binding British Journal of Pharmacology. ,vol. 154, pp. 872- 881 ,(2009) , 10.1038/BJP.2008.117
J. C. Henquin, H. P. Meissner, Effects of amino acids on membrane potential and 86Rb+ fluxes in pancreatic beta-cells American Journal of Physiology-endocrinology and Metabolism. ,vol. 240, ,(1981) , 10.1152/AJPENDO.1981.240.3.E245