Jab1 is a T2-dependent coactivator or a T3-dependent corepressor of TRB1-mediated gene regulation.

摘要: Thyroid hormones (THs) induce pleiotropic effects in vertebrates, mainly through the activation or repression of gene expression. These mechanisms involve thyroid hormone binding to receptors, an event that is followed by sequential recruitment coactivator corepressor proteins, which turn modify rate transcription. In present study, we looked for specific coregulators recruited long isoform teleostean receptor beta 1 (L-Trb1) when bound bioactive TH, 3,5-T2 (T2). We found jun domain-binding protein1 (Jab1) interacts with L-Trb1 + T2 complex. Using both and human TRB1 isoforms, characterized Jab1-TRB1 yeast two-hybrid, pull-down transactivation assays. Our results showed TRB1-Jab1 interaction was ligand dependent involved single Jab1 nuclear box, as well ligand-binding N-terminal domains TRB1. also provide evidence ligand-dependent, dual coregulatory properties Jab1. Indeed, T2 L-Trb1 hTRB1, acts a transcription, whereas it has activity interacting T3-bound S-Trb1 hTRB1. could explain some actions exerted THs regulate diverse biological processes.