Carbonic anhydrase: oxygen-18 exchange catalyzed by an enzyme with rate-contributing proton-transfer steps.
作者： David N. Silverman
DOI: 10.1016/S0076-6879(82)87037-7
关键词:
摘要: Publisher Summary This chapter reviews the application of an 18 O exchange method to study catalysis hydration CO 2 by carbonic anhydrase, which, with a maximal turnover number near 10 6 sec –1 , is one fastest known enzymic reactions. The so rapid that proton transfer steps between enzyme and its environment, which are not ordinarily rate limiting for slower reactions, become rate-limiting more amenable investigation anhydrase. chosen investigate this problem because it equilibrium may be used in absence buffer or presence excess concentration, buffers sources protons enzyme. With method, possible elucidate role pathway catalytic . A further result fate oxygen can followed; hence, reveals release from water bearing substrate oxygen, when O-labeled bicarbonate.
sci-hub.se 参考文章(25)
D. N. Silverman, C. K. Tu, Carbonic anhydrase catalyzed hydration studied by carbon-13 and oxygen-18 labeling of carbon dioxide Journal of the American Chemical Society. ,vol. 98, pp. 978- 984 ,(1976) , 10.1021/JA00420A019
C Tu, D N Silverman, G C Wynns, R E McMurray, CO2 kinetics in red cell suspensions measured by 18O exchange. Journal of Biological Chemistry. ,vol. 253, pp. 8178- 8184 ,(1978) , 10.1016/S0021-9258(17)34378-8
Raja G. Khalifah, The Carbon Dioxide Hydration Activity of Carbonic Anhydrase Journal of Biological Chemistry. ,vol. 246, pp. 2561- 2573 ,(1971) , 10.1016/S0021-9258(18)62326-9
Hakan STEINER, Bengt-Harald JONSSON, Sven LINDSKOG, The Catalytic Mechanism of Carbonic Anhydrase FEBS Journal. ,vol. 59, pp. 253- 259 ,(1975) , 10.1111/J.1432-1033.1975.TB02449.X
D. N. Silverman, C. K. Tu, Buffer dependence of carbonic anhydrase catalyzed oxygen-18 exchange at equilibrium Journal of the American Chemical Society. ,vol. 97, pp. 2263- 2269 ,(1975) , 10.1021/JA00841A045
George Hoch, Bessel Kok, A mass spectrometer inlet system for sampling gases dissolved in liquid phases. Archives of Biochemistry and Biophysics. ,vol. 101, pp. 160- 170 ,(1963) , 10.1016/0003-9861(63)90546-0
D. N. Silverman, C. K. Tu, S. Lindskog, G. C. Wynns, Rate of exchange of water from the active site of human carbonic anhydrase C Journal of the American Chemical Society. ,vol. 101, pp. 6734- 6740 ,(1979) , 10.1021/JA00516A040
H. Steiner, B.-H. Jonsson, S. Lindskog, The catalytic mechanism of human carbonic anhydrase C: Inhibition of CO2 hydration and ester hydrolysis by HCO−3 FEBS Letters. ,vol. 62, pp. 16- 20 ,(1976) , 10.1016/0014-5793(76)80006-3
C. K. Tu, D. N. Silverman, Mechanism of carbonic anhydrase studied by carbon-13 and oxygen-18 labeling of carbon dioxide Journal of the American Chemical Society. ,vol. 97, pp. 5935- 5936 ,(1975) , 10.1021/JA00853A060
R.H. Prince, P.R. Woolley, On the mechanism of action of carbonic anhydrase Bioorganic Chemistry. ,vol. 2, pp. 337- 344 ,(1973) , 10.1016/0045-2068(73)90034-5