Differential expression of microRNA (miRNA) in chordoma reveals a role for miRNA-1 in met expression

摘要: Emerging evidence suggests that microRNA (miRNA) expression signatures in cancer may have important diagnostic, prognostic, and therapeutic value, but there is no data on miRNA chordoma. The purpose of this study was to identify the role miRNAs human We analyzed chordoma-derived cell lines chordoma tissue by using microarray technology with unsupervised hierarchical clustering analysis. relative levels these were confirmed real-time quantitative RT-PCR Northern blot To characterize potential miRNA-1, miRNA-1 stably transfected into a line, UCH1. targeted gene Met tissues also studied. observe can be distinguished from normal muscle comparing profiles. Several differentially expressed compared controls, similar patterns found primary tissues. Importantly, we able show for first time, our knowledge, miRNA-206, two implicated number other types, markedly decreased both lines. When downregulation known targets observed. These included HDAC4-two genes observed overexpressed Our results demonstrate some are and, particular, functional effect tumor pathogenesis.