Eicosapentaenoic acid and docosahexaenoic acid modulate MAP kinase enzyme activity in human T-cells.

摘要: In order to investigate the implication of docosahexaenoic acid (DHA) and eicosapentaenoic (EPA) in T signalling, we assessed their effects on activation two mitogen activated protein (MAP) kinases, i.e. extracellularly-regulated kinases 1 2 (ERK1/ERK2) Jurkat T-cells. The n-3 polyunsaturated fatty acids (PUFAs) alone failed induce MAP kinase (MAPK) enzyme activity. To elucidate whether DHA EPA act via C (PKC) dependent independent pathways, employed respective activators, phorbol 12-myristate 13-acetate (PMA) antiCD3 antibodies. We observed that U0126, an inhibitor MAPK kinase-ERK 1/2 (MEK1/2), abolished actions these agents activation, suggesting they upstream MEK1/2. Further diminished both PMA- antibodies-induced activity ERK1/ERK2 Interestingly, okadaic (OA), a phosphatase seems downstream MEK1/2 as U0126 inhibit OA-induced activation. It is noteworthy not only curtail but also PUFAs at 20 μM potentiated action OA. Therefore, seem modulate On hand, arachidonic acid, n-6 PUFA conclusion, our study shows may regulate T-cells functions by modulating