The usefulness of immunohistochemistry in sporadic colorectal cancer.

摘要: Background: Colorectal cancer is an important disease with a large morbidity and mortality also increasing health care costs because of widespread the multi-modal therapy new drugs that continue to appear. There are 678.000 colorectal cases 400.000 deaths from worldwide. It second commonest cause death in European Union but, unlike related lung cancer, basis initiation currently not understood. At same time, incidence increases age, carcinomas being rare before age 40 years, excepting individuals genetic predisposition or predisposing conditions such as inflammatory bowel diseases. The early detection potential associated decrease mortality. Aim: Our study proposes find out significance some immunohistochemical markers (VEGF, p53, CK20 CEA) sporadic carcinoma establish statistical correlations between molecular tumor grade stage. Material Methods: We investigated histopathological inpatients (19 female 21 males) who undergone surgery for “Sf. Ioan” Emergency Hospital, Bucharest, September 2005–September 2006. proceeded examination grade, stage main features tumors, then we analyzed using ABC method immunohistochemistry following 20 selected cases: vascular endothelial growth factor (VEGF), carcinoembrionic antigen (CEA), cytokeratin (CK20), p53 oncoprotein. Finally, results t-Student test. Results: distribution (n = 40) regarding has showed preponderance patients older than 70 years (22/55%) small percentage younger adults (2/5%). repartition tumors sex ratio outlines difference males (21/52.5%), females (19/47.5%). analysis revealed high percent moderate (23/57.5%), comparison poor differentiated (11/27.5%) well-differentiated cancers (6/15%). neovascularity within stroma, growth, been noticed 15 (3.75%), lymphocyte infiltrate nine (22.5%). have positive correlation VEGF1 (r 0.4, p 0.05), CEA 0.88, 0.001). In addition, our demonstrate 0.43, 0.009), no relation among other markers. Conclusions: present shows immunostaining matter (100%). oncoprotein negative T1 T2 stages, but advanced stages half (50%). Regarding location, VEGF positively whatever topography. direct proportional expression CEA, p<0.001).