The Seed Region of a Small RNA Drives the Controlled Destruction of the Target mRNA by the Endoribonuclease RNase E

摘要: Numerous small non-coding RNAs (sRNAs) in bacteria modulate rates of translation initiation and degradation target mRNAs, which they recognize through base-pairing facilitated by the RNA chaperone Hfq. Recent evidence indicates that ternary complex Hfq, sRNA mRNA guides endoribonuclease RNase E to initiate turnover both RNAs. We show a not only defined site RNA, but also allosterically activates enzyme presenting monophosphate group at 5′-end cognate-pairing “seed.” Moreover, absence 5′-monophosphate makes seed region vulnerable an attack against Hfq confers no protection. These results suggest chemical signature pairing status may help ‘proofread’ recognition activate cleavage, as part dynamic process involving cooperation E.