The binding of high density lipoproteins to isolated rat hepatocytes.
作者： Leiv Ose , Ingeborg Røken , Kaare R. Norum , Christian A. Drevon , Trond Berg
DOI: 10.3109/00365518109092016
关键词:
摘要: The association of 125I-labelled rat high density lipoproteins (125I-HDL) to suspended hepatocytes was studied at 4°C. 125I-HDL associated isolated by two processes—one and one low affinity. cell-association 1251-HDL exhibited saturation kinetics inhibited varying degrees with both human such as VLDL, LDL HDL but not lipoprotein deficient serum or asialo-fetuin. did require divalent cations could be reduced pronase treatment the cells. binding site clearly different from receptor for in extrahepatic cells since heparin apolipoprotein E compete binding. Concanavalin A protein cholesterol ester labelled HDL. number sites 4°Cwas 2.2 × 106 per cell constant (Ka) 8.2 (mol/1) −1 Experiments [3H] means lecithin: ...
sci-hub.se 参考文章(55)
R W Mahley, T L Innerarity, R E Pitas, K H Weisgraber, J H Brown, E Gross, Inhibition of lipoprotein binding to cell surface receptors of fibroblasts following selective modification of arginyl residues in arginine-rich and B apoproteins. Journal of Biological Chemistry. ,vol. 252, pp. 7279- 7287 ,(1977) , 10.1016/S0021-9258(19)66966-8
Joseph L Goldstein, Michael S Brown, S. K. Basu, Characterization of the low density lipoprotein receptor in membranes prepared from human fibroblasts. Journal of Biological Chemistry. ,vol. 253, pp. 3852- 3856 ,(1978) , 10.1016/S0021-9258(17)34768-3
Claes-Henrik Florén, Åke Nilsson, Binding, interiorization and degradation of cholesterol ester-labelled chylomicron-remnant particles by rat hepatocyte monolayers Biochemical Journal. ,vol. 168, pp. 483- 494 ,(1977) , 10.1042/BJ1680483
T Nakai, P S Otto, D L Kennedy, T F Whayne, Rat high density lipoprotein subfraction (HDL3) uptake and catabolism by isolated rat liver parenchymal cells. Journal of Biological Chemistry. ,vol. 251, pp. 4914- 4921 ,(1976) , 10.1016/S0021-9258(17)33202-7
Mary Catherine Glick, Isolation of surface membranes from mammalian cells Mammalian Cell Membranes#R##N#General Concepts. pp. 45- 77 ,(1976) , 10.1016/B978-0-408-70722-0.50007-3
A Nilsson, Effects of anti-microtubular agents and cycloheximide on the metabolism of chylomicron cholesteryl esters by hepatocyte suspensions. Biochemical Journal. ,vol. 162, pp. 367- 377 ,(1977) , 10.1042/BJ1620367
Joseph L. Goldstein, Michael S. Brown, Binding and Degradation of Low Density Lipoproteins by Cultured Human Fibroblasts COMPARISON OF CELLS FROM A NORMAL SUBJECT AND FROM A PATIENT WITH HOMOZYGOUS FAMILIAL HYPERCHOLESTEROLEMIA Journal of Biological Chemistry. ,vol. 249, pp. 5153- 5162 ,(1974) , 10.1016/S0021-9258(19)42341-7
C.J. Fielding, I. Vlodavsky, P.E. Fielding, D. Gospodarowicz, Characteristics of chylomicron binding and lipid uptake by endothelial cells in culture. Journal of Biological Chemistry. ,vol. 254, pp. 8861- 8868 ,(1979) , 10.1016/S0021-9258(19)86779-0
Per O. Seglen, Preparation of isolated rat liver cells. Methods in Cell Biology. ,vol. 13, pp. 29- 83 ,(1976) , 10.1016/S0091-679X(08)61797-5
Gilbert Ashwell, Anatol G. Morell, The Role of Surface Carbohydrates in the Hepatic Recognition and Transport of Circulating Glycoproteins Advances in Enzymology - and Related Areas of Molecular Biology. ,vol. 41, pp. 99- 128 ,(2006) , 10.1002/9780470122860.CH3