Proteolytic 18O-labeling strategies for quantitative proteomics.
作者： Masaru Miyagi , K.C. Sekhar Rao
DOI: 10.1002/MAS.20116
关键词:
摘要: A number of proteomic techniques have been developed to quantify proteins in biological systems. This review focuses on the quantitative technique known as “proteolytic 18O-labeling.” utilizes a protease and H218O produce labeled peptides, with subsequent chromatographic mass spectrometric analysis identify (relative) from which peptides originated. The determines ratio individual protein's expression level between two samples relative each other, can be used quantitatively examine protein (comparative proteomics) post-translational modifications, study protein–protein interactions. present discusses various aspects 18O-labeling technique, including: its history, advantages disadvantages proteolytic compared other techniques, enzymatic considerations, problem variable incorporation 18O atoms into discussion recent advancements overcome it, computational tools interpret data, applications. © 2006 Wiley Periodicals, Inc., Mass Spec Rev 26:121–136, 2007
harvard.edu
sci-hub.se 参考文章(62)
K Rose, M G Simona, R E Offord, C P Prior, B Otto, D R Thatcher, A new mass-spectrometric C-terminal sequencing technique finds a similarity between γ-interferon and α2-interferon and identifies a proteolytically clipped γ-interferon that retains full antiviral activity Biochemical Journal. ,vol. 215, pp. 273- 277 ,(1983) , 10.1042/BJ2150273
Thomas P Conrads, Haleem J Issaq, Van M Hoang, Current Strategies for Quantitative Proteomics Proteome Characterization and Proteomics. ,vol. 65, pp. 133- 159 ,(2003) , 10.1016/S0065-3233(03)01018-0
DAVID B. SPRINSON, D. RITTENBERG, Nature of the Activation Process in Enzymatic Reactions Nature. ,vol. 167, pp. 484- 484 ,(1951) , 10.1038/167484A0
Jacob Bongers, Edgar P. Heimer, Recent applications of enzymatic peptide synthesis Peptides. ,vol. 15, pp. 183- 193 ,(1994) , 10.1016/0196-9781(94)90189-9
Michael J. MacCoss, Christine C. Wu, Dwight E. Matthews, John R. Yates, Measurement of the isotope enrichment of stable isotope-labeled proteins using high-resolution mass spectra of peptides. Analytical Chemistry. ,vol. 77, pp. 7646- 7653 ,(2005) , 10.1021/AC0508393
Dominic M. Desiderio, Masaaki Kai, Preparation of stable isotope-incorporated peptide internal standards for field desorption mass spectrometry quantification of peptides in biologic tissue Biological Mass Spectrometry. ,vol. 10, pp. 471- 479 ,(1983) , 10.1002/BMS.1200100806
Shao-En Ong, Matthias Mann, None, Mass spectrometry–based proteomics turns quantitative Nature Chemical Biology. ,vol. 1, pp. 252- 262 ,(2005) , 10.1038/NCHEMBIO736
Y. Oda, K. Huang, F. R. Cross, D. Cowburn, B. T. Chait, Accurate quantitation of protein expression and site-specific phosphorylation Proceedings of the National Academy of Sciences of the United States of America. ,vol. 96, pp. 6591- 6596 ,(1999) , 10.1073/PNAS.96.12.6591
Kris Gevaert, An Staes, Jozef Van Damme, Sara De Groot, Koen Hugelier, Hans Demol, Lennart Martens, Marc Goethals, Joël Vandekerckhove, Global phosphoproteome analysis on human HepG2 hepatocytes using reversed-phase diagonal LC. Proteomics. ,vol. 5, pp. 3589- 3599 ,(2005) , 10.1002/PMIC.200401217
Natascia Campostrini, Liliana B. Areces, Juri Rappsilber, Maria Chiara Pietrogrande, Francesco Dondi, Fabio Pastorino, Mirco Ponzoni, Pier Giorgio Righetti, Spot overlapping in two‐dimensional maps: A serious problem ignored for much too long Proteomics. ,vol. 5, pp. 2385- 2395 ,(2005) , 10.1002/PMIC.200401253