NG2‐positive cells generate A2B5‐positive oligodendrocyte precursor cells

摘要: Cellular specification of the oligodendrocyte lineage occurs through a series stages identified by expression distinct biochemical characteristics. The best characterized progenitor cell (OPC) in vitro is bipotential O2-A progenitor, labeling with monoclonal antibody A2B5, which proliferates predominantly response to platelet derived growth factor (PDGF). cellular ancestors cells are currently unclear. In vivo OPCs can be surface markers NG2 (a sulfated proteoglycan) and receptor alphaR). Substantial evidence supports generation oligodendrocytes from NG2(+), PDGFalphaR(+) both vitro. developmental relationship between NG2(+) A2B5(-) positive unknown it unclear whether they represent identical, partially overlapping or nonoverlapping populations cells. Here we show that cultures developing brain A2B5(+) arise populations. appear prior generate We propose during development NG2(+)/A2B5(-) (pre-OPCs) direct ancestor O2A (OPCs).