Quality cell therapy manufacturing by design

Elizabeth Csaszar, Kun Chen, Julia Caldwell, Warren Chan, Peter W. Zandstra, Real-time monitoring and control of soluble signaling factors enables enhanced progenitor cell outputs from human cord blood stem cell cultures. Biotechnology and Bioengineering. ,vol. 111, pp. 1258- 1264 ,(2014) , 10.1002/BIT.25163

Flavia Marturana, Nicholas E. Timmins, Lars K. Nielsen, Short-term exposure of umbilical cord blood CD34+ cells to granulocyte–macrophage colony-stimulating factor early in culture improves ex vivo expansion of neutrophils Cytotherapy. ,vol. 13, pp. 366- 377 ,(2011) , 10.3109/14653249.2010.518610

Shugo Tohyama, Fumiyuki Hattori, Motoaki Sano, Takako Hishiki, Yoshiko Nagahata, Tomomi Matsuura, Hisayuki Hashimoto, Tomoyuki Suzuki, Hiromi Yamashita, Yusuke Satoh, Toru Egashira, Tomohisa Seki, Naoto Muraoka, Hiroyuki Yamakawa, Yasuyuki Ohgino, Tomofumi Tanaka, Masatoshi Yoichi, Shinsuke Yuasa, Mitsushige Murata, Makoto Suematsu, Keiichi Fukuda, Distinct metabolic flow enables large-scale purification of mouse and human pluripotent stem cell-derived cardiomyocytes. Cell Stem Cell. ,vol. 12, pp. 127- 137 ,(2013) , 10.1016/J.STEM.2012.09.013

Uri Ben-David, Qing-Fen Gan, Tamar Golan-Lev, Payal Arora, Ofra Yanuka, Yifat S. Oren, Alicia Leikin-Frenkel, Martin Graf, Ralph Garippa, Markus Boehringer, Gianni Gromo, Nissim Benvenisty, Selective elimination of human pluripotent stem cells by an oleate synthesis inhibitor discovered in a high-throughput screen. Cell Stem Cell. ,vol. 12, pp. 167- 179 ,(2013) , 10.1016/J.STEM.2012.11.015

Emanuel J P Nazareth, Joel E E Ostblom, Petra B Lücker, Shreya Shukla, Manuel M Alvarez, Steve K W Oh, Ting Yin, Peter W Zandstra, High-throughput fingerprinting of human pluripotent stem cell fate responses and lineage bias. Nature Methods. ,vol. 10, pp. 1225- 1231 ,(2013) , 10.1038/NMETH.2684

B. Leobon, I. Garcin, P. Menasche, J.-T. Vilquin, E. Audinat, S. Charpak, Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 7808- 7811 ,(2003) , 10.1073/PNAS.1232447100

E. Ratcliffe, K.E. Glen, V.L. Workman, A.J. Stacey, R.J. Thomas, A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture. Journal of Biotechnology. ,vol. 161, pp. 387- 390 ,(2012) , 10.1016/J.JBIOTEC.2012.06.025

Christopher A Bravery, Jessica Carmen, Timothy Fong, Wanda Oprea, Karin H Hoogendoorn, Juliana Woda, Scott R Burger, Jon A Rowley, Mark L Bonyhadi, Wouter Van't Hof, None, Potency assay development for cellular therapy products: an ISCT review of the requirements and experiences in the industry. Cytotherapy. ,vol. 15, pp. 9- 19 ,(2013) , 10.1016/J.JCYT.2012.10.008

Mauro Krampera, Jacques Galipeau, Yufang Shi, Karin Tarte, Luc Sensebe, Immunological characterization of multipotent mesenchymal stromal cells--The International Society for Cellular Therapy (ISCT) working proposal. Cytotherapy. ,vol. 15, pp. 1054- 1061 ,(2013) , 10.1016/J.JCYT.2013.02.010

Jennifer Turner, Lake-Ee Quek, Drew Titmarsh, Jens O Krömer, Li-Pin Kao, Lars Nielsen, Ernst Wolvetang, Justin Cooper-White, None, Metabolic Profiling and Flux Analysis of MEL-2 Human Embryonic Stem Cells during Exponential Growth at Physiological and Atmospheric Oxygen Concentrations PLOS ONE. ,vol. 9, ,(2014) , 10.1371/JOURNAL.PONE.0112757