The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis.
作者： Brian S. DeDecker
DOI: 10.1007/978-1-4939-6343-0_10
关键词:
摘要: Current gene synthesis methods often incorporate a PCR-amplifying step in order to yield sufficient final product that is detectable and resolvable from multiple off-products. This amplification can cause stochastic sampling effects propagate errors during the lower variability when applied towards construction of randomized libraries. We present method for polymerase reaction (PSR), simple DNA polymerase-based assembles oligonucleotides unidirectional fashion without need PCR-type (Lee et al., BioTechniques 59:163-166, 2015). The PSR efficient with little off-product production, undetected effects, maximized used synthesize phage display
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