Comparative studies on synaptosomes: Applicability of the rapid method for preparing synaptosomes to elasmobranch brain.

摘要: A flotation technique for the rapid preparation of synaptosomes, originally developed invertebrate nervous tissue, has now been successfully applied to that an elasmobranch fish (Mustelis canis, dogfish). The involves submitting supernatant, obtained after a homogenate centrifuged at low speed remove nuclei and tissue debris centrifugal fields intermediate intensity (106g/min), appears separate well-sealed synaptosomes from those less well sealed as judged by criteria osmotic shrinkage, enzyme occlusion, choline uptake. do not equilibrate with 0.8 M sucrose used homogenization medium rise form coherent pellicle top tube. Due short (1–1/1/2 hr) time, such may prove useful in further metabolic studies.