Fatty acids stimulate cholecystokinin secretion via an acyl chain length-specific, Ca2+-dependent mechanism in the enteroendocrine cell line STC-1

作者: J. T. McLaughlin , R. B. Lomax , L. Hall , G. J. Dockray , D. G. Thompson

DOI: 10.1111/J.1469-7793.1998.011BY.X

关键词:

摘要: 1. The present study has investigated whether fatty acids directly influence peptide release from enteroendocrine cells using STC-1, a mouse intestinal endocrine tumour cell line, previously shown to cholecystokinin (CCK) in response other physiological stimuli. 2. Fatty elicited chain length- and dose-dependent stimulation of CCK secretion. Dodecanoic acid (C12) was most effective, producing up 5-fold increase with less than ten carbon atoms did not length dependence these effects mimics closely acid-induced secretion observed humans vivo. 3. Esterification C12 abolished secretion, indicating critical role for free carboxyl group eliciting In contrast, modification the methyl terminus had no effect on C12-induced non-metabolizable analogue 2-bromododecanoic equally effective. 4. marked intracellular calcium levels (200-300 nM) STC-1 which by L-type Ca2+ channel antagonist nicardipine. C8 produced smaller more transient response. also blocked 5. These data suggest that can interact stimulate via increases mediated primarily channels.

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