Noncontact three-dimensional mapping of intracellular hydromechanical properties by Brillouin microscopy
作者： Giuliano Scarcelli , William J Polacheck , Hadi T Nia , Kripa Patel , Alan J Grodzinsky
DOI: 10.1038/NMETH.3616
关键词:
摘要: Current measurements of the biomechanical properties cells require physical contact with or lack subcellular resolution. Here we developed a label-free microscopy technique based on Brillouin light scattering that is capable measuring an intracellular longitudinal modulus optical The 3D maps obtained in 2D and microenvironments revealed mechanical changes due to cytoskeletal modulation cell-volume regulation.
nature.com
core.ac.uk
harvard.edu
laserquantum.com参考文章(40)
Roger D. Kamm, Mohammad R. K. Mofrad, Cytoskeletal Mechanics: List of Contributors ,(2006) , 10.1017/CBO9780511607318
Cytoskeletal mechanics : models and measurements Cambridge University Press. ,(2006) , 10.1017/CBO9780511607318
E.L. Elson, T. Wakatsuki, N.C. Thompson, B. Schwab, Effects of cytochalasin D and latrunculin B on mechanical properties of cells Journal of Cell Science. ,vol. 114, pp. 1025- 1036 ,(2001) , 10.1242/JCS.114.5.1025
Giuliano Scarcelli, Roberto Pineda, Seok Hyun Yun, Brillouin optical microscopy for corneal biomechanics. Investigative Ophthalmology & Visual Science. ,vol. 53, pp. 185- 190 ,(2012) , 10.1167/IOVS.11-8281
Vesna Damljanovic, B. Christoffer Lagerholm, Ken Jacobson, Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays BioTechniques. ,vol. 39, pp. 847- 851 ,(2005) , 10.2144/000112026
D. E. Ingber, Tensegrity I. Cell structure and hierarchical systems biology. Journal of Cell Science. ,vol. 116, pp. 1157- 1173 ,(2003) , 10.1242/JCS.00359
G. Bao, S. Suresh, Cell and molecular mechanics of biological materials Nature Materials. ,vol. 2, pp. 715- 725 ,(2003) , 10.1038/NMAT1001
T. G. Mason, K. Ganesan, J. H. van Zanten, D. Wirtz, S. C. Kuo, Particle Tracking Microrheology of Complex Fluids Physical Review Letters. ,vol. 79, pp. 3282- 3285 ,(1997) , 10.1103/PHYSREVLETT.79.3282
Nadja Nijenhuis, Xuegen Zhao, Alex Carisey, Christoph Ballestrem, Brian Derby, None, Combining AFM and Acoustic Probes to Reveal Changes in the Elastic Stiffness Tensor of Living Cells Biophysical Journal. ,vol. 107, pp. 1502- 1512 ,(2014) , 10.1016/J.BPJ.2014.07.073
Ben Fabry, Geoffrey N. Maksym, James P. Butler, Michael Glogauer, Daniel Navajas, Jeffrey J. Fredberg, Scaling the microrheology of living cells. Physical Review Letters. ,vol. 87, pp. 148102- ,(2001) , 10.1103/PHYSREVLETT.87.148102