DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein
作者： Joan Boyes , Adrian Bird
DOI: 10.1016/0092-8674(91)90267-3
关键词:
摘要: We have studied the mechanism by which DNA methylation inhibits transcription both in cell-free nuclear extracts and living cell. Repression of vitro for four different promoters was shown to be an indirect effect. The mediator repression had properties indistinguishable from those a methyl-CpG binding protein (MeCP-1) that has been previously identified. Use differentially methylated competitors transient transfection assays suggested via MeCP-1 also occurs This supported fact MeCP-1-deficient cells showed much reduced genes.
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sci-hub.se 参考文章(37)
E. J. Murray, F. Grosveld, Site specific demethylation in the promoter of human gamma-globin gene does not alleviate methylation mediated suppression. The EMBO Journal. ,vol. 6, pp. 2329- 2335 ,(1987) , 10.1002/J.1460-2075.1987.TB02508.X
P Dobrzanski, A Hoeveler, W Doerfler, Inactivation by sequence-specific methylations of adenovirus promoters in a cell-free transcription system. Journal of Virology. ,vol. 62, pp. 3941- 3946 ,(1988) , 10.1128/JVI.62.11.3941-3946.1988
D.P. Bednarik, J.A. Cook, P.M. Pitha, Inactivation of the HIV LTR by DNA CpG methylation: evidence for a role in latency. The EMBO Journal. ,vol. 9, pp. 1157- 1164 ,(1990) , 10.1002/J.1460-2075.1990.TB08222.X
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
I. Kovesdi, R. Reichel, J. R. Nevins, Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 2180- 2184 ,(1987) , 10.1073/PNAS.84.8.2180
T Mohandas, R. Sparkes, L. Shapiro, Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation Science. ,vol. 211, pp. 393- 396 ,(1981) , 10.1126/SCIENCE.6164095
L. Venolia, S. M. Gartler, E. R. Wassman, P. Yen, T. Mohandas, L. J. Shapiro, Transformation with DNA from 5-azacytidine-reactivated X chromosomes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 79, pp. 2352- 2354 ,(1982) , 10.1073/PNAS.79.7.2352
F Watt, P L Molloy, Cytosine methylation prevents binding to DNA of a HeLa cell transcription factor required for optimal expression of the adenovirus major late promoter. Genes & Development. ,vol. 2, pp. 1136- 1143 ,(1988) , 10.1101/GAD.2.9.1136
Bernd Weisshaar, Klaus-Dieter Langner, Ruth Jüttermann, Ulrich Müller, Christiane Zock, Thomas Klimkait, Walter Doerfler, Reactivation of the methylation-inactivated late E2A promoter of adenovirus type 2 by E1A (13 S) functions Journal of Molecular Biology. ,vol. 202, pp. 255- 270 ,(1988) , 10.1016/0022-2836(88)90456-1
J. D. Dignam, R. M. Lebovitz, R. G. Roeder, Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei Nucleic Acids Research. ,vol. 11, pp. 1475- 1489 ,(1983) , 10.1093/NAR/11.5.1475