Differential sensitivity of human glioblastoma LN18 (PTEN-positive) and A172 (PTEN-negative) cells to Taxol for apoptosis

摘要: Glioblastoma is the most malignant brain tumor in humans and an average survival of glioblastoma patients hardly exceeds 12 months. Taxol a plant-derived anti-cancer agent, which has been used treatments many solid tumors. Deletion or mutation phosphatase tension homolog located on chromosome ten (PTEN) occurs more than 80% glioblastomas. We examined sensitivity human LN18 (PTEN-positive) A172 (PTEN-negative) cells to for induction apoptosis. Wright staining showed morphological features apoptosis after treatment with different doses 24 h. Significant amount occurred 25 nM Taxol, while only 50 Taxol. Western blotting antibody that could specifically detect activation phosphorylation Akt (p-Akt) did not show any p-Akt but increase cells. Activation be reversed by pre-treatment phosphatidylinositol-3-kinase (PI3K) inhibitor LY294002, indicating involvement PI3K activity this process. Apoptosis Bax:Bcl-2 mitochondrial release cytochrome c into cytosol leading mitochondria-dependent caspase cascade. cause upregulation vascular endothelial growth factor (VEGF), key mediator angiogenesis, substantial VEGF After increases maintain respectively, PTEN-negative glioblastoma. As single chemotherapy, might efficacious PTEN-positive Thus, our study differential