Mono-(2-Ethylhexyl) Phthalate (MEHP) Promotes Invasion and Migration of Human Testicular Embryonal Carcinoma Cells

摘要: Testicular dysgenesis syndrome refers to a collection of diseases in men, including testicular cancer, that arise as result abnormal development. Phthalates are class chemicals used widely the production plastic products and other consumer goods. Unfortunately, phthalate exposure has been linked reproductive dysfunction shown adversely affect normal germ cell In this study, we show mono-(2-ethylhexyl) (MEHP) induces matrix metalloproteinase 2 (MMP2) expression embryonal carcinoma NT2/D1 cells but no significant effect on MMP9 expression. also have higher levels MYC following MEHP treatment. It is recognized activation MMP2 tightly associated with tumor metastasis progression. Gelatin zymographic analysis indicates strongly activates cells. Addition MMP2-specific inhibitor SB-3CT inhibited MEHP-enhanced invasion migration, demonstrating plays functional role promoting progression response exposure. Furthermore, investigated genome-wide gene profiles at 0, 3, 24 h. Microarray semiquantitative RT-PCR revealed primarily influenced genes adhesion transcription Gap junction protein-alpha 1, vinculin, DNA-binding protein-1 were significantly down-regulated by treatment, while claudin-6 beta 1-catenin up-regulated. This study provides insight into mechanisms may account for modulating cancer